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Green Fluorescent Protein Purification
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Green Fluorescent Protein Purification
Green Fluorescent Protein (GFP) is a relatively new biochemical tool with a wide range of applications in current research. The natural function of GFP is to facilitate the bioluminescence of jellyfish and other coelenterates. Aequorea GFP (from jellyfish) consists of 238 amino acids packed tightl .... Middle of paper .... its compact beta-can structure. Biochemists now frequently exploit GFP’s unique structural and physical characteristics to visualize various cellular activities.
One such application of GFP is to understand the role of a hormone, T3, in the translocation of thyroid hormone nuclear receptors (TRs).* In this project, GFP was fused to the human TR subtype b1 protein and the protein’s intracellular movement was visually tracked in live cells in t ....
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Number of words: 1280 Number of pages: 5 (approx. 250 words per double-spaced page)
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