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Fluorescence Study Of Dyes
Beginning of paper
1. Introduction
Coumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to interpret and analyse the fluorescence spectra.
For fluorescence .... Middle of paper
.... distance at which decay and energy transfer are equally probable, as well as prove that Stern-Volmer's law and Förster's theory are obeyed.
2. Results
2.1. Electronic Absorption Spectra
We made up stock solutions for both salts and diluted them down to use in the UV/vis spectrometer.
Coumarin 1
C = 8 x10-5 mol dm -3
We obtained a spectrum with a maximum at 376.5nm at an absorbance of 1.4573, using Beer-Lambert's law, we dedu ....
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Number of words: 815
Number of pages: 3 (approx. 250 words per double-spaced page)
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